Fig. 2

The accelerated ovarian failure model of menopause induced glucose intolerance over time. At 7 months old (4.5 months post first 4-VCD or vehicle injection), glucose intolerance was assessed with a GTT following a 16 h fast (A). Glucose clearance was gauged by concentrations of glucose in the blood measured over time (time 0 = fasting blood glucose). B Blood glucose concentration over time was used to calculate area under the curve (AUC). Glucose clearance (C) and intolerance (D, AUC) was also assessed at 8.5 months old (6 months post first 4-VCD or vehicle injection). Data are presented as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, 2-way ANOVA with Tukey’s multiple comparison test, (n = 10–13/group)