Fig. 2

White matter changes are driven by VCID in males and HF diet in females. Representative images of myelin basic protein (MBP) labeling (top panel) and CC1 labeling (mid panel) are shown in A for the corpus callosum (CC) and F for the hippocampus. In the merged image shown in the bottom panel, MBP is shown in magenta, CC1 in cyan and DAPI in blue. Scale bar 100 µm. Labeling density quantification in the CC (B, C) and the hippocampus (G, H). Data are represented as mean + SEM. 2-way ANOVA with Tukey’s post hoc test ^#p < 0.05, ^##p < 0.01 effect of VCID; *p < 0.05, **p < 0.01 effect of diet. 3-way ANOVA results are reported under the graph *p < 0.05. Correlations of glucose intolerance (GTT-AUC) with MBP (D, I) and CC1 (E, J) densities were analyzed using simple linear regression for males and females combined and for each sex separately with r² and p values noted for each. n = 3–6 mice/group. Ctrl control diet, HFD high fat diet