Fig. 2

Fluorescent-Labeled EV Uptake by Cells. A Confocal fluorescent microscopy image of PKH26-labeled EVs taken up by naïve NSCs; PKH26-labeled EVs are red and NSC nuclei counterstained with DAPI are blue. B Flow cytometric analysis for proportion of label-positive NSCs in unstained control cells, cells directly labeled with PKH26, cells that have sequestered labeled EVs, and cells that were incubated with purified culture medium spiked with PKH26 dye, that were then processed for the same labeling and filtration protocol as that used for isolated EVs; n = 4 to 7 samples per group; Kruskal–Wallis test for nonparametric one-way ANOVA, approximate p-value < 0.0001 followed by Dunn’s multiple comparisons post hoc test, *p < 0.05, **p < 0.01