Fig. 1

Lipidomic workflow, describing the subjects analyzed and comparisons performed at each step. A Human and murine experimental groups. B After EV isolation from human and mouse blood plasma, lipids were extracted for quantification and identification by LC–MS/MS. Additional exploratory and differential lipid abundance analyses were also performed. After lipid class annotation, a class enrichment analysis was also carried out for human samples. Finally, functional profiling was applied to interpret the differential abundance analysis results