Fig. 3

Cpt1a ablation in AgRP neurons reduces lipid content in white adipose tissue. a, b Representative image of gonadal white adipose tissue (gWAT) (a) and inguinal white adipose tissue (iWAT) (b) of female and male mice vs their control littermates. c, j Average adipocyte area of gWAT in female (c, n = 10–10) and male mice (j, n = 10–15). d, k Morphometric analysis of the adipocyte area distribution in the gWAT of female (d, n = 5–5) and male mice (k, n = 5–5). g, n Average adipocyte area of iWAT in female (g, n = 10–10) and male mice (n, n = 8–8). h, o Morphometric analysis of adipocyte area distribution in the iWAT of female (h, n = 5–5) and male mice (o, n = 5–5). e, f TG and NEFAs measurement in female (n = 5–5) and male mice (n = 5–5) (l–m). i, p Analysis by qRT-PCR of the mRNA levels of Pnpla2, Leptin, Ucp1, Cpt1a, Il6, Adiponectin and Resistin in the iWAT of female (i, n = 8–7) and male mice (p, n = 6–6). Data are expressed as the mean ± SEM. In d, h, k, o, *p < 0.05, **p < 0.001, ****i < 0.0001, using two-way repeated-measures ANOVA followed by Šidák’s post hoc test. In c, e, f, g, i, j, l, m, n, p, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using Student’s t-test