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Fig. 2 | Biology of Sex Differences

Fig. 2

From: CPT1A in AgRP neurons is required for sex-dependent regulation of feeding and thirst

Fig. 2

Cpt1a ablation in AgRP neurons increases brown adipose tissue activity. a, b EE profile normalized against the lean body mass in female (a, n = 6–6) and male mice (b, n = 6–6). c Representative infrared thermal images of female (top panel, n = 11–9) and male mice (bottom panel, n = 8–7). d Quantification of the interscapular temperature adjacent to the brown adipose tissue (BAT) of female (n = 11–9) and male mice (n = 8–7). e, f Weight of BAT normalized against the body weight of female (e, n = 6–9) and male mice (f, n = 6–7). g Representative images of BAT sections dyed with the H&E stain in female (top panel) and male mice (bottom panel). Scale bar, 100 μm (×magnification 20). h, i Lipid droplet quantification using ImageJ in female (h, n = 4–4) and male mice (i, n = 4–4). j, k Analysis by qRT-PCR of the mRNA levels of Pnpla2, Lipe, Cpt1b, Slc2a4 Ucp1, Cidea, Mmp2, Leptin, Adiponectin and Resistin in female (j, n = 7–7) and male mice (k, n = 5–6). l, m Representative western blot of UCP1 protein levels in BAT (30 μg) from female (l, n = 6–8) and male mice (m, n = 6–8) normalized against β-actin levels. Data are expressed as the mean ± SEM. In d * p < 0.05, ***p < 0.001, using two-way ANOVA followed by Turkey post hoc test. In a, b, e, f, h, I, j–m, *p < 0.05, **p < 0.01, using Student’s t-test

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