Fig. 3

Quantitative RT-PCR analysis of ASD-related TFs in the hippocampus of offspring prenatally exposed to BPA or vehicle control. The expression levels of eight ASD-related TFs (i.e., Ar, Esr1, Egr2, Kdm5b, Sox5, Smad4, Tcf7l2, and Yy1) whose targets were differentially expressed in response to BPA were analyzed by qRT-PCR analysis using the hippocampus of pups prenatally exposed to BPA (n = 6 pups; 3 males and 3 females from 3 different dams) or vehicle control (n = 6 pups; 3 males and 3 females from 3 different dams). Data are presented as the mean ± SEM. The differences between the two groups were analyzed using the two-tailed Student’s t test. A p-value < 0.05 was considered significant. *p value < 0.05