Fig. 3
From: Sex differences in hearing impairment due to diet-induced obesity in CBA/Ca mice
Cochlear hair cells are intact throughout all treatment groups. Whole-mount preparations of the auditory epithelium in the female (a–c) and male (d–f) mice. Tissues were stained for myosin VIIa (red, hair cell marker) and phalloidin (green, filamentous actin, F-actin, marker), then photographed using epifluorescence. No hair cell loss was observed on IHCs and OHCs: apex, middle, and basal turns. Scale bar = 50 μm. g, h OHCs (g) and IHCs (h) remained intact in all cochlear turns. Two-way ANOVA; Tukey's multiple comparisons test; all comparisons, p > 0.05