Fig. 7
Estrogen treatment reduces eosinophils and neutrophils in the BAL of OVX-OVA-challenged mice. Animals were prepared as in Fig. 1. A Flow cytometry gating strategy for eosinophils defined as live, singlet, CD11b+Siglec-F+CD11c− cells is shown, while neutrophils are defined as FSCloSSChiCD11b+CD11c+Siglec-Flo. B Total CD45+ BAL cells, C eosinophil and D neutrophils per mL of lavage fluid are shown for all groups. E Total CD45+ cells detected in the remaining lung tissue F eosinophils and G neutrophils were quantified. Bar graphs show the data as mean ± SEM (n = 5–6 mice/group). The data represent 4 independent experiments. Statistical significance was determined as in Fig. 3. * Indicates p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001