Fig. 6

Circulating cytokines and chemokines are selectively altered with steady-state estrogen treatment. Groups of mice were prepared as in Fig. 1. Circulating cytokines were determined by ELISA in serum that was diluted 1:5 for all groups. A IL-5 and B IL-13 cytokines were detected, C the neutrophil activation marker, MPO, and D–F circulating chemokines D CCL22, E CCL3 and F KC are shown. The data are shown as mean ± SEM. Data are representative of 4 independent experiments. Statistical significance was determined as before with a p < 0.05 (indicated by *), **p < 0.01, ***p < 0.001, ****p < 0.0001