Fig. 4

Total immune cell infiltrate is reduced in BAL fluid from OVX-E2-OVA-treated animals compared to F-INTACT, OVA-treated. Animals were treated as in Fig. 1. Cytospins were prepared from BAL fluids collected immediately following methacholine challenges. Total BAL return volume and numbers of total cells (B) were recorded and applied to cytospin slides at 300×g for 10 min. Slides were stained with Giemsa and 2–4 fields were assessed per slide. 200 cells per field were counted as C macrophages, D neutrophils, E eosinophils or F lymphocytes. A Percentages of each cell population are shown. B–F Are the counts of cells determined by multiplying the percentages by the total number of cells recovered in each lavage. Data are representative of 3 independent experiments with 3–8 animals per group. One-way ANOVA was used to determine statistical differences across all groups; Tukey’s post-test were used to determine statistical differences between groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001