Fig. 2

Linear regression of T cell abundances (CD8 + and CD4 + FoxP3 +) quantified by quantitative immunofluorescence (QIF) against relative abundance of bile acids linked to poorer clinical outcomes. A Representative right-sided colon tumor tissue stained and imaged, blue = DAPI; nuclei, green = cytokeratin, yellow = CD8 + T cells, magenta = CD4 + T cells, Red = FoxP3 + T cells, scale bar 100 μM. B Representative histopathologic assigned normal colon tissue from a right-sided colon cancer patient, blue = DAPI; nuclei, green = cytokeratin, yellow = CD8 + T cells, magenta = CD4 + T cells, Red = FoxP3 + T cells, scale bar 100 μM. C Correlation between CD4 + FoxP3 + and glycine-conjugated bile acids stage II tumors (n = 10). D Correlation between CD4 + FoxP3 + and glycine-conjugated bile acids stage II right-sided colon tumors (n = 6). * P < 0.05, 95% confidence bands shown