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Fig. 4 | Biology of Sex Differences

Fig. 4

From: Myocytic androgen receptor overexpression does not affect sex differences in adaptation to chronic endurance exercise

Fig. 4

HSAAR Tg genotype affects glycolytic myofiber size but not proportion, while sex affects both glycolytic and oxidative myofiber size and proportion across EDL. Visualization of a succinate dehydrogenase (SDH) stained, hematoxylin and eosin (H&E) matched transverse photomicrographs of extensor digitorum longus (EDL) at 40× magnification, b quantification of minor ellipse of oxidative SDH-stained H&E-matched myofibrils, c quantification of minor ellipse of glycolytic SDH-nonstained H&E-matched myofibrils, d proportion of oxidative (SDH-stained) myofibrils between male and female rats across 40× magnified photomicrographs (collapsed by genotype and exercise), e proportion of glycolytic (SDH-nonstained) myofibrils between HSAAR Tg and WT rats across 40× magnified photomicrographs (collapsed by sex and exercise). Photomicrographs (a) represent between-group subjects across SDH and H&E staining after the experimental paradigm. Indicated groups (b, c): males (yellow), females (green), sedentary (grey), aerobic exercise (red), wild-type (wt) (blank fill), and HSAAR transgenic (Tg) (hatch fill). Data (b, c) are presented as means ± s.e.m, with n = 6 per sex–genotype–exercise group. Data (d, e) are presented as frequency distributions. d n = 24 per sex (collapses n = 6 transgene–exercise, n = 6 wild-type–exercise, n = 6 transgene–sedentary, n = 6 wild-type–sedentary). e n = 24 per genotype (collapses n = 6 female–exercise, n = 6 male–exercise, n = 6 female–sedentary, n = 6 male–sedentary). All data analyzed using three-way ANOVA with post hoc Tukey HSD for multiple comparisons. Scale bar, 100 µm

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