Fig. 3

The effect of sex and HSAAR Tg genotype on glycolytic and oxidative muscle fiber-type expression and canonical mitochondrial biogenesis protein marker expression. Quantification of western blot analysis of a, e myosin heavy chain 2a (MHC2a), b, f myosin heavy chain 1 (MHC1), c, g peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α), and d, h mitochondrial transcription factor A (TFAM) from tibialis anterior (a–d) and soleus (e–h) muscles. Representative immunoblots for tibialis anterior (a–d) and soleus (e–h) are shown between males and females, with their respective exercise and genotype groups. Indicated groups: males (yellow), females (green), sedentary (grey), aerobic exercise (red), wild-type (wt) (blank fill), and HSAAR transgenic (Tg) (hatch fill). Data expressed in relative density (R.D.) and presented as means ± s.e.m. Data analyzed using three-way ANOVA for (a–h). b–d n = 5 per sex–genotype–exercise group, a, e–h n = 4 per sex–genotype–exercise group