Fig. 8

Effect of EMPA on mRNA expression on major components of fatty acid oxidation in white adipose depots in PCOS. Effect of EMPA on subcutaneous white adipose tissue (WAT) mRNA expression of A carnitine palmitoyltransferase 1A (CPT1A), B carnitine palmitoyltransferase 1B (CPT1B), and C medium-chain acyl-CoA dehydrogenase (MCAD); retroperitoneal WAT mRNA expression of D CPT1A, E CPT1B, and F MCAD; and mesenteric WAT mRNA expression of G CPT1A, H CPT1B, and I MCAD after 3 weeks of EMPA treatment. Expression was normalized by the geometric mean of three housekeeping genes (GMHK) and standardized to untreated control rats. Log2 values are expressed as mean ± SEM. Data were analyzed by two-way ANOVA followed by Tukey post hoc tests. Significant interaction was only observed for MCAD in subcutaneous WAT. n = 7–8 per group