Fig. 2

Effect of EMPA on adipocyte cross-sectional area in white adipose depots in PCOS. Effect of EMPA on the relative frequency on adipocyte areas and average adipocyte area in A subcutaneous white adipose tissue, B visceral (retroperitoneal), and C visceral (mesenteric) white adipose tissue. Subcutaneous, retroperitoneal, and mesenteric adipocytes were binned every 400, 150, and 200 microns, respectively. Data were analyzed by two-way ANOVA followed by Tukey post hoc tests. Significant interactions were observed in all three depots with adipocyte area frequency, but there were no significant interactions with average adipocyte cross-sectional area in any of the three depots. *P < 0.05 compared to controls, ^#P < 0.05 CON + EMPA compared to PCOS + EMPA, ^&P < 0.05 PCOS compared to PCOS + EMPA. n = 8–10 per group