Table 1 Primer sequences
Protein name | Gene symbol | Forward primer | Reverse primer |
|---|---|---|---|
Leptin receptor | Lepr | AGCTAGGTGTAAACTGGGACA | GCAGAGGCGAATCATCTATGAC |
Adiponectin receptor protein 1 | AdipoR1 | TGGTCTTCGGGATGTTCTTC | CCCTGAATAGTCCAGTTTGGAA |
Platelet glycoprotein 4 | Cd36 | TTGAAAAGTCTCGGACATTGAG | TCAGATCCGAACACAGCGTA |
Solute carrier family 2, facilitated glucose transporter member 4 | Slc2a4 | ACACTGGTCCTAGCTGTATTCT | CCAGCCACGTTGCATTGTA |
Insulin receptor | Insr | TCAAGACCAGACCCGAAGATT | TCTCGAAGATAACCAGGGCATAG |
Insulin receptor substrate 1 | Irs1 | CGATGGCTTCTCAGACGTG | CAGCCCGCTTGTTGATGTTG |
Tumor necrosis factor | Tnf | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
Interleukin-1 beta | Il1b | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
Transforming growth factor beta-1 | Tgfb1 | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
Interleukin-10 | Il10 | CAGAGCCACATGCTCCTAGA | TGTCCAGCTGGTCCTTTGTT |
Heat shock factor protein 1 | Hsf1 | GGGAAACAGGAGTGTATGGACT | CTTGTTGACAACTTTTTGCTGCT |
Heat shock protein beta-1 | HspB1 | ATCCCCTGAGGGCACACTTA | GGAATGGTGATCTCCGCTGAC |
Alpha(B)-crystallin | HspB5 | GTTCTTCGGAGAGCACCTGTT | GAGAGTCCGGTGTCAATCCAG |
Heat shock protein beta-3 | HspB3 | AGACCCCAGTGCGTTATCAG | GCAGTGCGTATAGTGTATGATCC |
Heat shock protein beta-6 | HspB6 | TGTCCACGGACTCTGGGTATT | CACATGGTCGTCAACCACCTT |
Heat shock protein beta-8 | HspB8 | AGACCCCTTTCGGGACTCA | GGCTGTCAAGTCGTCTGGAA |
Heat shock protein 40 | Hsp40 | TTCGACCGCTATGGAGAGGAA | CACCGAAGAACTCAGCAAACA |
Heat shock protein 70 | Hsp70 | GAGATCGACTCTCTGTTCGAGG | GCCCGTTGAAGAAGTCCTG |
Carboxyl-terminus of Hsp70 Interacting Protein | Chip | CGGCAGCCCTGATAAGAGC | CACAAGTGGGTTCCGAGTGAT |
Bcl-2-associated athanogene 1 | Bag-1 | GCAGCAGGGAGTTGACTAGAA | TTACTTCCTCGGTTTGGGTCG |
Glucose-regulated protein 94 | Grp94 | AAGAATGAAGGAAAAACAGGACAAAA | CAAATGGAGAAGATTCCGCC |
C/EBP homologous protein | Chop | CCACCACACCTGAAAGCAGAA | AGGTGAAAGGCAGGGACTCA |
Binding immunoglobulin protein | Bip | TTCAGCCAATTATCAGCAAACTCT | TTTTCTGATGTATCCTCTTCACCAGT |
X-box-binding protein 1 total | Xbp1 total | TGGCCGGGTCTGCTGAGTCCG | GTCCATGGGAAGATGTTCTGG |
X-box-binding protein 1 unspliced | Xbp1us | CAGCACTCAGACTATGTGCA | GTCCATGGGAAGATGTTCTGG |
X-box-binding protein 1 spliced | Xbp1s | CTGAGTCCGAATCAGGTGCAG | GTCCATGGGAAGATGTTCTGG |
Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | GGGTTCCTATAAATACGGACTGC | CCATTTTGTCTACGGGACGA |