Fig. 4

Q-PCR validation of candidate microRNAs in maternal serum in uncomplicated and FGR pregnancies. qPCR was performed on individual microRNAs isolated from maternal serum of women with appropriately grown (AGA; IBC 20–80th) or growth-restricted (FGR; IBC < 5th) infants using specific primers for A miR-28-5p, B miR-29c-3p, C miR-301a-3p, D miR-378a-3p, E miR-409-3p, F miR-454-3p and G miR-526b-5p. Data were normalised to 2 reference miRNAs and expressed as 2^−ΔCt. Individual data points shown (n = 18–31/group); line represents the median. *p < 0.05 Mann–Whitney U test