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Fig. 3 | Biology of Sex Differences

Fig. 3

From: Sex differences in the expression of the endocannabinoid system within V1M cortex and PAG of Sprague Dawley rats

Fig. 3

Immunohistochemical analysis of the AEA system. A Immunohistochemistry of naïve male and female cortex. Staining for NAPE-PLD, FAAH, Iba1, GFAP, and Nissl was performed. Original magnification ×63. B Immunohistochemistry of naïve male and female PAG. Staining for NAPE-PLD, FAAH, Iba1, GFAP, and Nissl was performed. Original magnification ×63. Scale on lower left image applies to all frames. C Measurement of relative area of IHC field occupied by NAPE-PLD staining pixels for each sex and region. No significant differences were observed between region or sex. D Measurement of co-efficient of colocalization for NAPE-PLD with IBa1, Nissl, and GFAP. NAPE-PLD colocalized with Nissl at significantly higher rates in female cortex vs male cortex (****p = 0.004). No significant differences observed in PAG. E Measurement of relative area of IHC field occupied by FAAH staining pixels for each sex and region. FAAH immunoreactivity was significantly higher in male cortex vs female cortex (****p = 0.003). F Measurement of co-efficient of colocalization for FAAH with IBa1, Nissl, and GFAP. No significant differences observed between region or sex. All calculations for co-efficient of colocalization were performed with respect to the relevant enzyme. n = 3/sex for each analysis

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