Fig. 3
Myocardial fibrosis 16 weeks after cardiac-specific Ampkα2 deletion in male and female mice. A Representative pictures of fibrosis analysis by Sirius red staining of subequatorial heart section (right panels), proportion of total fibrosis in males and females (upper right panel) and % of total, perivascular and interstitial fibrosis in males (lower right panel). B Left ventricular mRNA expression level of collagen 1 (Col1a1) and collagen 3 (Col3a1). C Correlation curve between ejection fraction and total fibrosis in males. (n = 5 to 7 per experimental group). ANOVA: §p < 0.05 for the genotype effect; µµ p ≤ 0.01 for the sex effect; $p < 0.05 for interaction; Post hoc Tukey test: *p < 0.05, **p < 0.01, ***p < 0.001 Ampkα2kf/f vs Ampkα2ciKO (same sex); ¤p < 0.05 males vs females (same genotype)