Fig. 4

RUPP rats present with larger glomeruli in rat kidneys. Paraffin-embedded rat kidneys were cut at 10-μm sections and stained with H&E staining to visualise tissue morphology. a Images of entire tissue sections were taken at × 4, × 10 and × 20 objective using an Axioscan microscope to produce virtual slides of sham and RUPP kidneys. Scalebar = 20 μm. b Average glomerular area was measured at a × 20 magnification using ZEN Lite 3.2 software. Data plotted as mean ± SEM; n = 7; unpaired Student’s t-test; *p < 0.05