Fig. 2

RUPP hearts express higher mRNA and protein levels of FKBPL. Total RNA extracted from rat hearts by TRIsure reagent was analysed by RT-qPCR to determine mRNA expression levels of Fkbpl, Flt1 and Vegfa. Relative mRNA expression of a Fkbpl, b Flt1 and c Vegfa mRNA levels comparing sham and RUPP hearts normalized to B-actin. Data presented as mean fold change ± SEM; n ≥ 6; Mann-Whitney t-test; *p < 0.05. d Fkbpl expression was verified at the protein level by enzyme-linked immunosorbent assay (ELISA), concentration calculated against a known standard and plotted against total mg of protein per sample. Data presented as mean ± SEM; n ≥ 6; unpaired Student’s t-test. e, f Cardiac FKBPL expression was further analysed by immunofluorescent staining and intensity measured using ImageJ by analysing greyscale value in six × 40 images per animal. Image brightness was adjusted equally for representative images displayed. Data plotted as mean fold change ± SEM; n ≥ 5; unpaired Student’s t-test; *p < 0.05