Fig. 9

Androgen receptor degradation blocked testosterone-induced neurodegeneration in N27 cells. Cells were seeded for 24 h, followed by J9 pretreatment for 30 min. At 80% confluency, cells were exposed to H₂O₂ for 1 h followed by testosterone treatment for 2 h. J9 blocked further exacerbation of H₂O₂-induced cell loss in N27 cells (a). However, J9 attenuated H₂O₂-induced cell loss in C6 cells, and thus, its effect on testosterone could not be determined (b). Results were determined by ANOVA followed by Fisher LSD post hoc test. Results are reported as mean + SEM. p < 0.05; *versus control, **versus H₂O₂. C, vehicle control; H, H₂O₂; T, 100 nM testosterone; H, H₂O₂; HT, post-treatment T; J9, ASC J9