Fig. 4

Neuroprotection is via estrogen receptor. 17β-estradiol and testosterone pretreatment prior to oxidative stressor is neuroprotective, regardless of oxidative stress insult (a, b). Estrogen receptor antagonist, ICI, was able to block 17β-estradiol’s neuroprotective action, but did not impact H₂O₂-induced cell loss (c). Results were determined by ANOVA followed by Fisher LSD post hoc test. Results are reported as mean + SEM. p < 0.05; *versus control, **versus H₂O₂. C, vehicle control; E, 1 nM 17β-estradiol; H, H₂O₂; EH, pre-treatment E; ICI, estrogen receptor antagonist. T, 100 nM testosterone; TH, pre-treatment T