Fig. 2

An effect of hormone pretreatment in N27 and C6 cells in the presence of oxidative stress. Hormone alone has no effect on cell viability. H₂O₂ decreased the cell viability by ~ 20–30%. Testosterone and 17β-estradiol pretreatment prior to H₂O₂ is protective in N27 cells (a, b) and not in C6 cells (d, e). DHT-BSA, followed by H₂O₂ exposure, did not protect N27 and C6 cells in an oxidative stress environment (c, f). Results were determined by ANOVA followed by Fisher LSD post hoc test. Results are reported as mean + SEM. p < 0.05; *versus control, **versus H₂O₂. C, vehicle control; T, 100 nM testosterone; H, H₂O₂; TH, pre-treatment T. E, 1 nM 17β-estradiol; EH, pre-treatment E; D, 500 nM DHT-BSA; DH, pre-treatment D