Fig. 9

Transgene expression reduced Ih current in a sex-dependent manner in mice aged of 20 months. a An example of recorded neuron following a voltage step, from − 60 to − 100 mV. Application of ZD7288 (20 μM), an antagonist of the hyperpolarized-activated current Ih [57], in the same neuron showed its slow and persistent activation in EC. b Ih was measured by subtracting the current before and after its slow and persistent activation, as illustrated by the line with two arrows. Illustrations of Ih currents generated by a voltage step, from − 60 to − 100 mV in males (c) and females (d), both transgenic or NonTg animals. e Ih current generated by hyperpolarizing voltage steps was decreased by 3xTg-AD expression in females, whereas the reduction was not significant in males. The number of mice included in each group was 4 for NonTg males, 5 for 3xTg-AD males, 8 for NonTg females, and 4 for 3xTg-AD females. Statistical comparisons were performed using unpaired Student’s t test (− 80 to − 100 mV) or Welch’s t test (− 70 mV). *p < 0.05