Fig. 4

Transgene expression changed the passive properties of EC deep-layer neurons only in 20-month-old females. a Electrical representation of a cell membrane. b To quantify passive properties, different intensities of hyperpolarizing current were injected into a neuron in current clamp: voltage variation (V) and time constant (T) were measured after each injection. The panel illustrated on one trace the electrical properties measured for the calculation of passive properties. c The resting potential was not influenced by transgenes or sex. The expression of AD-related transgenes increased input resistance (d) and reduced Gc (e) in neurons of female animals, but not in males. (f) CC of neurons in NonTg females was higher than these of 3xTg-AD females and NonTg males. The number of mice included in each group was 4 for NonTg males, 5 for 3xTg-AD males, 8 for NonTg females, and 4 for 3xTg-AD females. Statistical comparisons were performed using unpaired Student’s t tests (c, d, and e) or Welch’s t tests (f). Abbreviations: CC, cell capacitance; Gc, cell conductance. *p < 0.05