Fig. 11

Sex-dependent alteration of paired-pulse ratio from intracortical synaptic transmission by 3xTg-AD expression in mice aged of 20 months. a, b Evoked excitatory and inhibitory input has been discriminated by generating postsynaptic currents at different imposed voltages. The excitatory inputs produced a depolarizing current that increases with hyperpolarization of resting potential, whereas inhibitory inputs generated both hyperpolarizing (− 50 mV and -60 mV) and depolarizing currents (− 70 mV), depending on whether imposed voltage was under or over the reversal potential of Cl⁻ ions, estimated at − 63 mV. There are examples of eEPSC recordings for a paired electrical stimulation (interval of 100 ms) in males (c) and females (d), both transgenic or NonTg animals. e The P2/P1 ratio was decreased by transgene expression in male, but not in female mice. f The application of the GABA_a receptor antagonist picrotoxin (100 μM) and the NMDA receptor antagonist D-APV (100 μM) did not affect the amplitude or the kinetic of eEPSC, showing that these receptors did not play a significant part in it. The number of mice included in each group was 3 for NonTg males, 4 for 3xTg-AD males, 4 for NonTg females, and 4 for 3xTg-AD females. Statistical comparisons were performed using unpaired Student’s t test. Abbreviations: eEPSC, evoked postsynaptic current. *p < 0.05