Fig. 2

HELLP and ischemia reperfusion (IR) during pregnancy increase renal injury. a–d Representative pictures from kidneys collected at gestational day 19 (GD19; scale bar is 500 μM). Kidney sections were stained with Mason’s trichrome, and blue areas indicate increased fibrosis (e; n = 6/group with 10 images per animal scored). A separate group of rats (n = 4/group) had renal function assessed by FITC-sinistrin GFR at GD19, and HELLP+AKI rats had significantly less GFR relative to NP rats (f). Urinary KIM-1 (g) and NGAL (h) excretion was increased in response to IR indicative of AKI (n = 10/group). Data is expressed as mean ± SEM. *^–***p < 0.05–p < 0.0005 between indicated groups. Black arrows are used to point out areas of increased blue staining