Table 1 Gene primers for RT-PCR
Gene | Sequence |
|---|---|
Angiotensinogen (Agt) | Sense: AGCACGGACAGCACCCTATT |
Antisense: AGAACTCATGGAGCCCAGTCA | |
Angiotensin-converting enzyme (ACE) | Sense: CTGCCTCCCAACGAGTTAGAA |
Antisense: CGGGACGTGGCCATTATATT | |
Angiotensin II type I receptor (AT1R) | Sense: TATCACAGTGTGCGCGTTTCA |
Antisense: TGGTAAGGCCCAGCCCTAT | |
Renin | Sense: GCTACATGGAGAATGGGACTGAA |
Antisense: ACCACATCTTGGCTGAGGAAAC | |
Reference gene: elongation factor-1 (Ef-1) | Sense: GCAAGCCCATGTGTGTTGAA |
Antisense: TGATGACACCCACAGCAACTG |