Fig. 7

Gender disparity in metabolic pathways and biomarkers during hepatocarcinogenesis in Hras12V transgenic mice. a The mRNA levels of Cyp3a41 in W, P, and T of males and females as detected by RT-qPCR. The mRNA levels of the genes were normalized to Rp135a. The data are expressed as the mean ± SEM (n = 5–7). b The expression levels of key enzymes in terpenoid backbone biosynthesis and steroid biosynthesis pathways identified by TMT-based quantitative proteomic analysis. c The expression levels of SAA2, ORM2, and SERPINA1E identified by TMT-based quantitative proteomic analysis. *p < 0.05; **p < 0.01. The abbreviation and symbol definitions, as well as descriptions, are the same as those in the caption for Fig. 1