Fig. 2
From: Unfractionated heparin displaces sFlt-1 from the placental extracellular matrix
Flt fluorescence. BeWos cultured on 4-well chamber slides with or without 10 U/mL unfractionated heparin were probed for Flt (which includes both Flt-1 and sFlt-1) using a fluorescent antibody and visualized using an EVOS. Quantification of average fluorescence (a) and representative images (b) are presented. Hoescht, blue channel, indicates nuclei, while sFlt-1 fluorescence is shown in green channel. Scale bar represents 200 microns. N = 10–12 per group. Statistical analysis performed using unpaired Student’s t test. Significance is designated by a bar connecting groups (*p < 0.05, **p < 0.01, ***p < 0.001)