Fig. 1

RNAseq of rat developmental time points near male sex determination. a Principle components analysis of global gene expression measured by RNAseq done in two experiments (blue = experiment 1 at Hudson Alpha Institute of Biotechnology HAIB, peach = experiment 2 at UCLA). ts, tail somites. b Plot of genes in experiment 1 differentially expressed between E12–13 vs E11 (x-axis) and E12–13 vs E14 (y-axis). Values in blue were for those genes from experiment 1 with differences that in experiment 2 (21 ts, 21 tail somites) had expression values below 1 TPM (transcript per million reads sequenced) and those in red with values > 10 TPM. Three clusters were noted (1–3) with the most significant genes listed in order for each group shown below the chart. c Transcripts per million reads sequenced (TPM) for genes known to be involved in testis or ovary differentiation. Experiment 1 values are shown in gray and experiment 2 in red. Error bars represent the standard deviation