Effects of fasting on the expression pattern of FGFs in different skeletal muscle fibre types and sexes in mice

Jia, Wei-hua; Wang, Nuo-qi; Yin, Lin; Chen, Xi; Hou, Bi-yu; Wang, Jin-hua; Qiang, Gui-fen; Chan, Chi Bun; Yang, Xiu-ying; Du, Guan-hua

doi:10.1186/s13293-020-00287-7

Biology of Sex Differences

Table 1 Primers used for real-time PCR

From: Effects of fasting on the expression pattern of FGFs in different skeletal muscle fibre types and sexes in mice

Pairs	Genes	Primer sequence
1	Mouse Fgf1 5′	CCCTGACCGAGAGGTTCAAC
1	Mouse Fgf1 3′	GTCCCTTGTCCCATCCACG
2	Mouse Fgf2 5′	GCGACCCACACGTCAAACTA
2	Mouse Fgf2 3′	TCCCTTGATAGACACAACTCCTC
3	Mouse Fgf3 5′	TACAACGCAGAGTGTGAGTTTG
3	Mouse Fgf3 3′	CACCGACACGTACCAAGGTC
4	Mouse Fgf4 5′	TACCCCGGTATGTTCATGGC
4	Mouse Fgf4 3′	TTACCTTCATGGTAGGCGACA
5	Mouse Fgf5 5′	AAGTAGCGCGACGTTTTCTTC
5	Mouse Fgf5 3′	CTGGAAACTGCTATGTTCCGAG
6	Mouse Fgf6 5′	CAGGCTCTCGTCTTCTTAGGC
6	Mouse Fgf6 3′	AATAGCCGCTTTCCCAATTCA
7	Mouse Fgf7 5′	CTCTACAGATCATGCTTCCACC
7	Mouse Fgf7 3′	ACAGAACAGTCTTCTCACCCT
8	Mouse Fgf8 5′	AGAGCCTGGTGACGGATCA
8	Mouse Fgf8 3′	CTTCCAAAAGTATCGGTCTCCAC
9	Mouse Fgf9 5′	ATGGCTCCCTTAGGTGAAGTT
9	Mouse Fgf9 3′	TCCGCCTGAGAATCCCCTTT
10	Mouse Fgf10 5′	TTTGGTGTCTTCGTTCCCTGT
10	Mouse Fgf10 3′	TAGCTCCGCACATGCCTTC
11	Mouse Fgf11 5′	TAGCCTGATCCGACAGAAGC
11	Mouse Fgf11 3′	GGCAGAACAGTTTGGTGACG
12	Mouse Fgf12 5′	CAGGCCGTGCATGGTTTCTA
12	Mouse Fgf12 3′	TCGTGTAGTGATGGTTCTCTGT
13	Mouse Fgf13 5′	CTCATCCGGCAAAAGAGACAA
13	Mouse Fgf13 3′	TTGGAGCCAAAGAGTTTGACC
14	Mouse Fgf14 5′	CCCCAGCTCAAGGGCATAG
14	Mouse Fgf14 3′	TGATGGGTAGAGGTAACCTTCTC
15	Mouse Fgf15 5′	ATGGCGAGAAAGTGGAACGG
15	Mouse Fgf15 3′	CTGACACAGACTGGGATTGCT
16	Mouse Fgf16 5′	GTGTTTTCCGGGAACAGTTTGA
16	Mouse Fgf16 3′	GGTGAGCCGTCTTTATTCAGG
17	Mouse Fgf17 5′	GGCAGAGAGCGAGAAGTACAT
17	Mouse Fgf17 3′	CGGTGAACACGCAGTCTTTG
18	Mouse Fgf18 5′	CCTGCACTTGCCTGTGTTTAC
18	Mouse Fgf18 3′	TGCTTCCGACTCACATCATCT
19	Mouse Fgf20 5′	GGTGGGGTCGCACTTCTTG
19	Mouse Fgf20 3′	GATACCGAAGAGACTGTGATCCT
20	Mouse Fgf21 5′	CTGCTGGGGGTCTACCAAG
20	Mouse Fgf21 3′	CTGCGCCTACCACTGTTCC
21	Mouse Fgf22 5′	CCAGGACAGTATAGTGGAGATCC
21	Mouse Fgf22 3′	AGTAGACCCGCGACCCATAG
22	Mouse Fgf23 5′	ATGCTAGGGACCTGCCTTAGA
22	Mouse Fgf23 3′	AGCCAAGCAATGGGGAAGTG
23	Mouse Flrt2 5′	ATGGGCCTACAGACTACAAAGT
23	Mouse Flrt2 3′	CAGCGGCATACACTAGGGC
24	Mouse Flrt3 5′	CCTCATCGGGACTAAAATTGGG
24	Mouse Flrt3 3′	GCAAGTTCTTCAAATCGGAAGGA
25	Mouse Tbp 5′	ACCCTTCACCAATGACTCCTATG
25	Mouse Tbp 3′	ATGATGACTGCAGCAAATCGC

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ISSN: 2042-6410

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