Fig. 9

Flutamide reduced systolic blood pressure and vascular hypertrophy in castrated mice treated with 6β-hydroxytestosterone (6β-OHT). Castrated Cyp1b1^+/+ mice were infused with vehicle or angiotensin (Ang) II (700 ng/kg/day) and treated with 6β-OHT(15 μg/g b.w every third day) and flutamide (FLU) (8 mg/kg every day), and blood pressure was measured (a). H&E staining was performed to determine vascular hypertrophy, and the media to lumen ratio was calculated (b). The graph depicts the quantified data. *P < 0.05 Cas+6β-OHT, Cas+6β-OHT+FLU vs. corresponding values from Ang II-treated animals; ^†P < 0.05 Cas+ 6β-OHT+FLU+Ang II vs. Cas+ 6β-OHT+Ang II (n = 5 for all experiments, two-way ANOVA; data are expressed as mean ± SEM)