Fig. 8

6β-Hydroxytestosterone (6β-OHT) restored angiotensin (Ang) II-induced superoxide production that was minimized in castrated (Cas) Cyp1b1^+/+and Cyp1b1^−/− mice. Cyp1b1^+/+ and Cyp1b1^−/− mice were castrated and then infused with vehicle or Ang II (700 ng/kg/day) (upper panel) and treated with 6β-OHT (15 μg/g b.w every third day) or 6β-OHT+Ang II (lower panel) for 14 days. a Aortic superoxide production was determined by the fluorescence intensity of 2-hydoxyethidium. a, b Photomicrographs are representative of the aorta from mice in each of the different treatment groups following incubation with dihydroethidium. b The graph depicts the quantified data. *P < 0.05 vehicle vs. corresponding values from Ang II-treated animals (n = 3 for all experiments, unpaired t test, and the data are expressed as mean ± SEM)