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Fig. 6 | Biology of Sex Differences

Fig. 6

From: 6β-Hydroxytestosterone, a metabolite of testosterone generated by CYP1B1, contributes to vascular changes in angiotensin II-induced hypertension in male mice

Fig. 6

Castration (Cas) mitigated angiotensin (Ang) II-induced vascular fibrosis in Cyp1b1+/+ mice, which was restored by 6β-hydroxytestosterone (6β-OHT). Cyp1b1+/+ and Cyp1b1−/− mice were castrated and infused with Ang II (700 ng/kg/day) and treated with 6β-OHT (15 μg/g b.w every third day). At the end of Ang II infusion, the aortas were removed, cut into sections, and stained with Masson’s trichrome staining for localization of collagen deposition (a, b). The percentage positive area for collagen staining was calculated. *P < 0.05 Cas + 6β-OHT vs. corresponding values from Ang II-treated animals (n = 3 for all experiments, two-way ANOVA; data are expressed as mean ± SEM)

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