Table 2 rhAMP-SNP-assay sequences. Each rhAMP-assay consists of a set of allele-specific primers to distinguish between both alleles as well as a locus-specific primer common to both allelic variants. 5-prime modifications to each allele-specific primer regarding FAM/VIC-based fluorescence detection and 3-prime “blocking” immediately downstream of the given ribonucleotide remain subject to the vendor’s discretion (IDT)
Assay name | Allele-specific primer 1 | Allele-specific primer 2 | Locus-specific primer |
|---|---|---|---|
SNP_A | CCCCGAGCCAAAGATG | CCCCCGAGCCAAAGATA | GCGGATTGAGGAGAATGTGGGA |
SNP_B | ATTCAGAGCGGCACC | CATTCAGAGCGGCACT | GCGACCTTGTCTGCCAATATCC |
SNP_C | AGAGAAACACCACAAATGATATCG | CAGAGAAACACCACAAATGATATCA | GCATCGTGTTCCAGCTGCTT |