Fig. 5
From: Cardiac hypertrophy with obesity is augmented after pregnancy in C57BL/6 mice
mRNA abundance of key genes regulating extracellular matrix and cardiac hypertrophy in left ventricles of low fat- and high-fat-fed nulliparous and postpartum mice. a mRNA abundance of genes regulating the extracellular matrix (ECM): Col1a1, Col3a1, Mmp9, Mmp3, and Timp1. b Ratios of Timp1to Mmp3 and Mmp9. c mRNA abundance of fetal gene program genes, Acta1, Myh6, and Myh7. d Ratio of Myh6 to Myh7, a marker of fetal gene reactivation. e mRNA abundance of natriuretic peptides A and B (Nppa and Nppb). f mRNA abundance of estrogen receptors α and β (Esr1 and Esr2). mRNA abundance of genes from left ventricles of low-fat (LF)– and high-fat (HF)–fed nulliparous (NP) and postpartum (P) mice was quantified using a custom CodeSet from NanoString and analyzed on an nCounter Analysis System. Data are expressed as counts of mRNA transcripts, normalized to the geometric mean of counts of four housekeeping genes (Gapdh, Eef1e1, Rpl4, and Ywhaz). A complete list of genes included in the custom CodeSet is available in Additional file 1: Table S2. Data are mean + SEM from n = 9 (LF, NP, and P), n = 7 (HF, NP), and n = 11 (HF, P). *P < 0.05 compared with LF using 2-way ANOVA followed by Holm-Sidak pairwise analysis. #P < 0.05 compared with NP using 2-way ANOVA followed by Holm-Sidak pairwise analysis