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Fig. 4 | Biology of Sex Differences

Fig. 4

From: Cardiac hypertrophy with obesity is augmented after pregnancy in C57BL/6 mice

Fig. 4

Cardiac fibrosis with high-fat feeding is not augmented in postpartum mice. a mRNA abundance of fibrosis-related genes (Map 3k7, Tgfb3, Tgfbr2, Tgfbr3, and Smad2) in nulliparous (NP) and postpartum (P) mice fed a low-fat (LF) and high-fat (HF) diet for 20 weeks. mRNA abundance of genes left ventricles was quantified using a custom CodeSet from NanoString and analyzed on an nCounter Analysis System. Data are expressed as counts of mRNA transcripts, normalized to the geometric mean of counts of four housekeeping genes (Gapdh, Eef1e1, Rpl4, and Ywhaz). A complete list of genes included in the custom CodeSet is available in Additional file 1: Table S2. Data are mean + SEM from n = 9 (LF, NP, and P), n = 7 (HF, NP), and n = 11 (HF, P). b Representative images (× 40) of picrosirius red–stained sections and c quantification of interstitial collagen content in the LV wall of NP- and P HF-fed mice. Data are mean + SE from the average of 5 fields per section (3 sections per mouse, and n = 3 mice per group). *P < 0.05 compared with LF using 2-way ANOVA followed by Holm-Sidak pairwise analysis

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