Fig. 4

Comparison of protein expression in spleen tissue from female and male mice. A quantitative analysis of western blot (WB) images obtained from 1, 5, and 10-month old female (F) and male (M) mice showing the relative amounts of Complex I (a), Complex II (b), ATP synthase (c), VDAC1 (d), UCP2 (e), and β-actin (f) as compared to a spleen tissue standard (Std). Representative WB images are shown below the plots. 50 μg of total protein was loaded in each lane. Values represent the means ± SD of data obtained from six animals per group; *p < 0.05, **p < 0.01, and ***p < 0.001 (mark age differences); #p < 0.05, ##p < 0.01, and ###p < 0.001 (mark sex differences)