Fig. 2

Comparison of protein expression in brown adipose tissue (BAT) from female and male mice. A quantitative analysis of western blot (WB) images obtained from 1, 5, and 10-month old female (F) and male (M) mice showing the relative amounts of Complex I (a), Complex II (b), ATP synthase (c), VDAC1 (d), UCP1 (e), UCP3 (f), and α-actin (g) as compared to a BAT tissue standard (Std). Representative WB images are shown below the plots. 20 μg of total protein were loaded in each lane. Values represent the means ± SD of data obtained from six animals per group; *p < 0.05, **p < 0.01, and ***p < 0.001 (mark age differences); #p < 0.05, ##p < 0.01, and ###p < 0.001 (mark sex differences)