Fig. 4

Senescent cell burden is higher in PE-MSC compared to NP-MSC. SABG staining revealed a higher number of stained cells (marked with black arrows) in PE-MSC compared to their normotensive counterparts (a). Data presented as mean values of SABG-stained MSC with min-max (b). Expression of p16, but not p21, was significantly increased. All SASP genes were significantly more highly expressed in PE-MSC compared to NP-MSC. Data are shown as box-plots (min-max) with all individual values (c). PE-MSC and NP-MSC were co-cultured with GFP expressing HUVEC for 8 days in total, and total network length was measured continuously every 3 h. Significantly lower angiogenic potential was registered for HUVEC co-cultured with PE-MSC, compared to NP-MSC (F = 13.965; df = 8, p < 0.001) (d)