Table 1 Oligonucleotides used for quantitative RT-PCR. Official gene symbols, NCBI accession number of targeted transcripts, and forward and reverse oligonucleotide sequences are depicted
From: Sexual dimorphism in PAR2-dependent regulation of primitive colonic cells
Genes | NCBI accession number | Forward Reverse |
|---|---|---|
F2rl1 (PAR2) | NM_007974.4 | GGACCGAGAACCTTGCAC GAACCCCTTTCCCAGTGATT |
F2r (PAR1) | NM_010169.4 | CAGCCAGAATCAGAGAGGACAGA TGTATTTTCACTGGGATTCCTTAGAA |
Ctnnb1 | NM_007614.3 NM_001165902.1 | GCTATTCCACGACTAGTTCAG GGAATGGTATTGAGTCCTCG |
Adam10 | NM_007399.4 | GAAGATGGTGTTGCCGACAG TTTCCATACTGACCTCCCAGC |
Cd44 | NM_009851.2 NM_001039150.1 NM_001177785.1 NM_001177786.1 NM_001177787.1 | TCTGCCATCTAGCACTAAGAGC GTCTGGGTATTGAAAGGTGTA |
Cd24a | NM_009846.2 | GGCACTGCTCCTACCCACGC CACCCCCTCTGGTGGTAGCG |
Dclk1 | NM_019978 | CTGCAGCAGGAGTTTCTGTA CCGAGTTCAATTCCGGTGGA |
Myc | NM_010849.4 NM_001177353.1 | AGTGCTGCATGAGGAGACAC GCCTCTTCTCCACAGACACC |
Ccnd1 | NM_007631.2 | TGCGTGAGAAGGAGATTGT CTTCGCACTTCTGCTCCTCA |
Rhob | NM_007483 | TGACTTGGGGTCGAGAGGAA AAAAGTGACCCCACTGCACA |
Mapk3 | NM_011952.2 | CACTGGCTTTCTGACGGAGT GGATTTGGTGTAGCCCTTGGA |
Sox9 | NM_011448 | GAGCCGGATCTGAAGAGGGA GCTTGACGTGTGGCTTGTTC |
Timp1 | NM_001044384.1 NM_011593.2 NM_001294280.2 | GAGCCCTGCTCAGCAAAGAG GGACCTGATCCGTCCACAAAC |
Itga3 | NM_013565.3 NM_001306162.1 | GATTCCTGGTGGTGAAGGAGG GGGACACAGGTACACAGCAC |
Dusp6 | NM_026268.3 | CAAGCAAATTCCTATCTCGG GTCGTAAGCATCGTTCATG |
Itga6 | NM_008397.4 NM_001277970.1 | CTCCCTCTCAGACTCGGTCA CTGGCGGAGGTCAATTCTGT |
Timp2 | NM_011594.3 | CAACAGGCGTTTTGCAATGC ATCCTCTTGATGGGGTTGCC |
Gsk3b | NM_019827.7 NM_001347232.1 | TGGTGCTGGACTATGTTC GTTCTGTGGTTTAATGTCTCG |
Hprt1 | NM_013556 | TCAGTCAACGGGGGACATAAA GGGGCTGTACTGCTTAACCAG |
Gapdh | NM_001289726 | AGGTCGGTGTGAACGGATTTG TGTAGACCATGTAGTTGAGGTCA |