Fig. 2

Cell proliferation in colonoids from male and female mice and impact of PAR₂ expression. a Ki-67 labeling in male and female colonoids at day 6 of culture. Left panel: The percentage of Ki-67 positive nuclei was calculated as described in the “Methods” section by the ratio of positive Ki-67 nuclei vs total nuclei in the larger diameter of colonoids. Right panel: Representative colonoid labeling of Ki-67 (green), CD44 (red, immaturity marker), and nuclei by DAPI (cyan) is shown. b Comparative Ki-67 labeling in PAR₂ WT and PAR₂ KO male and female colonoids at day 6 of culture. Right panel: Representative PAR₂ KO colonoid labeling of Ki-67 (green), CD44 (red, immaturity marker), and nuclei by DAPI (cyan) is shown. Data are mean ± SEM of 6 (a) or 5 (b) colonoids from male or female mice from n = 2 independent experiments