Fig. 1
From: Adipocyte deficiency of ACE2 increases systolic blood pressures of obese female C57BL/6 mice
Development of a mouse model of adipocyte ACE2 deficiency. a Schematic representation depicting the loxP-flanked ACE2 allele before (a) and after successive recombination with Flp (b) and transgenic adiponectin-driven Cre expression (c). The disrupted allele is shown in c, indicating deletion of exon 4 of the ACE2 gene. b Tissue characterization demonstrating reduced ACE2 mRNA abundance is specific to adipose tissues (subcutaneous, SubQ; retroperitoneal, RPF) (n = 4–8 male mice/genotype). Data are mean + SEM; P < 0.05 compared to Ace2fl/y using t test. c PCR reactions were performed with DNA extracted from RPF (n = 3 female mice/genotype). Primers amplify a 923 base pair product for the disrupted portion of the ACE2 gene