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Table 1 Flow cytometry reagent list for the first dataset

From: An inherent T cell deficit in healthy males to C. neoformans infection may begin to explain the sex susceptibility in incidence of cryptococcosis

Antibody/Probe

Cell expression

Fluorochrome

Emission spectra (nm)

Vendor

Anti-human CD3

Pan expression T cell surface marker

APC

650/660

BD Biosciences (Franklin Lakes, NJ)

Anti-human CD4

Helper T cell surface marker

PE

496/578

BD Biosciences

Anti-human CD8

Cytotoxic T cell surface marker

FITC

494/520

BD Biosciences

Anti-human CD19

Pan expression B cell surface marker

PC7

496/785

Beckman Coulter (Brea, CA)

Live-dead discriminator

Compromised cell membrane marker

7AAD

546/647

Thermo Fisher (Waltham, MA)

  1. CD cluster of differentiation, APC allophycocyanin, PE phycoerythrin, FITC fluorescein isothiocyanate, PC7 phycoerythrin cyanine 7, 7AAD 7-aminoactinomycin D