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Table 1 Flow cytometry reagent list for the first dataset

From: An inherent T cell deficit in healthy males to C. neoformans infection may begin to explain the sex susceptibility in incidence of cryptococcosis

Antibody/Probe Cell expression Fluorochrome Emission spectra (nm) Vendor
Anti-human CD3 Pan expression T cell surface marker APC 650/660 BD Biosciences (Franklin Lakes, NJ)
Anti-human CD4 Helper T cell surface marker PE 496/578 BD Biosciences
Anti-human CD8 Cytotoxic T cell surface marker FITC 494/520 BD Biosciences
Anti-human CD19 Pan expression B cell surface marker PC7 496/785 Beckman Coulter (Brea, CA)
Live-dead discriminator Compromised cell membrane marker 7AAD 546/647 Thermo Fisher (Waltham, MA)
  1. CD cluster of differentiation, APC allophycocyanin, PE phycoerythrin, FITC fluorescein isothiocyanate, PC7 phycoerythrin cyanine 7, 7AAD 7-aminoactinomycin D