Fig. 2

Angiotensin-(1-7) does not alter fasting glucose or insulin levels. Circulating glucose and insulin levels were measured after a 4-h fasting period in control diet and high-fat diet (HFD)-induced obese male and female mice chronically treated with angiotensin (Ang)-(1-7) or saline (n = 8–12/group). a, b HFD tended to produce mild hyperglycemia, which was not different between sexes and not significantly affected by chronic Ang-(1-7) infusion. c, d HFD produced hyperinsulinemia in both sexes, but to a greater extent in male mice. There was no effect of Ang-(1-7) infusion on insulin levels. Data are mean ± SEM and were analyzed by multiple regression for main effects of sex (P_Sex), diet (P_Diet), and drug (P_Drug) and their pairwise interactions (P_Drug:Sex, P_Diet:Sex, and P_Drug:Diet)