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Table 2 Fatty acid profile (FAME measured by GC-FID) in liver, gAT and iAT

From: Sex-specific lipid molecular signatures in obesity-associated metabolic dysfunctions revealed by lipidomic characterization in ob/ob mouse

 

Fatty acids

F

M

 

P values

LIVER

Total C16

34.5 ± 0.8

32.9 ± 0.3

 

ns

Total C18

63.6 ± 0.3

64.7 ± 0.6

 

ns

Total C20

1.2 ± 0.4

1.8 ± 0.3

 

ns

C18:0/C16:0

0.02 ± 0.01

0.09 ± 0.01

**

P < 0.01

C18:1/C18:0

146 ± 46

25 ± 3

*

P < 0.05

C16:0/C18:2

10.2 ± 0.8

4.3 ± 0.6

**

P < 0.01

Total SFA (%)

27.2 ± 0.4

27.5 ± 0.5

 

ns

Total MUFA (%)

69.3 ± 0.2

65.4 ± 1.2

*

P < 0.05

Total PUFA (%)

3.0 ± 0.3

7.2 ± 0.8

**

P < 0.01

MUFA/SFA

2.5 ± 0.0

2.4 ± 0.1

 

ns

PUFA/SFA

0.1 ± 0.0

0.3 ± 0.0

**

P < 0.01

MUFA/PUFA

22.4 ± 1.9

9.6 ± 1.3

**

P < 0.01

gAT

Total C16

53.2 ± 3.6

43.8 ± 0.8

*

P < 0.05

Total C18

45.9 ± 3.4

55.2 ± 0.9

*

P < 0.05

C16:1/C16:0

0.46 ± 0.03

0.35 ± 0.02

*

P < 0.05

Total SFA (%)

37.1 ± 3.2

33.3 ± 0.4

 

ns

Total MUFA (%)

45.5 ± 1.2

45.2 ± 1.5

 

ns

Total PUFA (%)

18.8 ± 0.4

21.3 ± 1.6

 

ns

MUFA/SFA

1.3 ± 0.1

1.4 ± 0.1

 

ns

PUFA/SFA

0.6 ± 0.0

0.6 ± 0.1

 

ns

MUFA/PUFA

2.5 ± 0.1

2.2 ± 0.2

 

ns

iAT

Total C16

42.2 ± 4.4

35.3 ± 2.4

 

ns

Total C18

55.0 ± 3.7

66.0 ± 2.3

*

P < 0.05

C16:1/C16:0

0.49 ± 0.03

0.42 ± 0.08

 

ns

Total SFA (%)

30.2 ± 3.4

26.0 ± 0.7

 

ns

Total MUFA (%)

50.3 ± 2.2

48.0 ± 2.7

 

ns

Total PUFA (%)

18.5 ± 0.8

28.5 ± 2.0

*

P ≤ 0.01

MUFA/SFA

1.7 ± 0.2

1.8 ± 0.1

 

ns

PUFA/SFA

0.6 ± 0.1

1.1 ± 0.1

*

P < 0.05

MUFA/PUFA

2.7 ± 0.1

1.7 ± 0.2

*

P ≤ 0.01

  1. Data are presented as mean ± sem, n = 3–4. Differences between sexes were determined by two-tailed Student’s t test with statistical significance determined using the Holm-Sidak method, with alpha = 5.000%. Each row was analyzed individually, without assuming a consistent SD. *, P < 0.05, **, P < 0.01, M vs F were considered significant. ns = not significant. M male, F female, SFA saturated fatty acid, MUFA monounsaturated fatty acid, PUFA polyunsaturated fatty acid, gAT perigonadal adipose tissue and iAT inguinal adipose tissue