Fig. 2

Sex-dependent inflammatory response to obesity in liver, gAT and iAT. Representative histological sections of staining for F4/80 (n = 4) and relative mRNA expression level of inflammatory genes in a liver b gAT and c iAT; and b gAT and c iAT quantification of crown like structures in F (♀ - open bars and pink bullets) and M (♂ gray bars and blue triangles) mice (n = 7). Values are mean ± sem, n = 6–9; *, P < 0.05, **, P < 0.01, ***, P < 0.001 and ****, P < 0.0001 M vs F were considered significant. Tnfα tumor necrosis factor alfa, Il interleukin, Clec4f C-type lectin domain family 4 member F, Cd68 cluster of differentiation 68, Ccl chemokine (C-C motif) ligand and Mcp Monocyte chemoattractant protein